Fig. 7

Silencing Wnt5a suppresses M2-like TAM-mediated tumor growth and metastasis in vivo. a-c The morphological characteristics of tumor xenograft, tumor size and tumor weight in the HCT116 alone, HCT116 + sh-NC TAM and HCT116 + sh-Wnt5a TAM groups. Error bars, SEM. d Relative mRNA expression of Wnt5a, CD163 and IL-10 of tumors in different groups. Error bars, SEM. e IHC analyzed the expression of Wnt5a, CD163 and IL-10 protein of tumors in different groups. Bar = 50 μm. f Western blot analysis of Wnt5a, IL-10, p-CaMKII, p-ERK1/2, and p-STAT3 expression in different groups. g IHC analyzed Ki67 expression of tumors in different groups. Bar = 50 μm. Error bars, SEM. h Representative CTC images of immunofluorescence. Bar = 20 μm. i Quantification of CTCs captured from the blood of mice. Error bars, SEM. j Representative H&E stained sections of metastatic nodules in liver and lung tissue from mice in the HCT116+ sh-NC TAM group were shown. Bar = 200 μm. k Quantification of mice with metastasis lesions in different groups. Statistical analysis was conducted using one-way ANOVA. *P < 0.05. **P < 0.01. ***P < 0.001