Fig. 1

The ratio of Wnt5a⁺CD68⁺/CD68⁺ TAMs is correlated with poor prognosis in CRC patients. a Representative immunofluorescence staining images for Wnt5a (green), CD68 (red), DAPI (blue) in CRC samples. Bar = 100 μm. b Wnt5a⁺CD68⁺/CD68⁺ TAMs ratio was significantly elevated in primary human CRC tissues compared with normal colorectal tissues. Statistical analysis was conducted using one-way ANOVA. c, d Association of Wnt5a⁺CD68⁺/CD68⁺ TAMs ratio with recurrence-free survival and overall survival of CRC patients. e Representative immunofluorescence staining images for Wnt5a (green), CD68 (red), DAPI (blue) at tumor invasive front. Bar = 100 μm. f Wnt5a⁺CD68⁺/CD68⁺ TAMs ratio at tumor invasive front and tumor nest in 10 CRC samples. g Representative immunofluorescence photographs for co-localization staining of Wnt5a, M2 maker (CD163) and M1 maker (HLA-DR). Bar = 100 μm. Error bars, SEM. ***P < 0.001