Fig. 3

Both l-CDL and anti-NGF mAbs inhibited the expression of p-TAK1 in the DRG and sciatic nerve, and l-CDL induced antinociception and inhibition of p-TAK1 was reversed by intraplantar administration of NGF. a, b The mechanical and thermal thresholds of CCI rats after intraplantar administration of NGF (0.1 μg/30 μL) and intramuscular injection of l-CDL (6 mg/kg) around the sciatic nerve on the operated side of CCI rats (NGF was administered 15 min before l-CDL treatment). Vehicle: corn oil. n = 6 per group; ###P < 0.001, compared with l-CDL group; ***P < 0.001, compared with 0 h. The expression of p-TAK1 in DRG in CCI rats after intramuscular injection of l-CDL (6 mg/kg) around the sciatic nerve (c) and intraplantar administration of anti-NGF Abs (6 μg/30 μL) (d) or coadministration of l-CDL (6 mg/kg) with NGF (0.1 μg/30 μL) (e). The expression of p-TAK1 in the sciatic nerve in CCI rats after intramuscular injection of l-CDL (6 mg/kg) around the sciatic nerve (f) and intraplantar administration of anti-NGF Abs (6 μg/30 μL) (g) or coadministration of l-CDL (6 mg/kg) and NGF (0.1 μg/30 μL) (h). n = 4 per group; #P < 0.05, and ##P < 0.01, compared with the control group; *P < 0.05, and **P < 0.01, compared with the CCI group