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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Regulators of G-protein signaling, RGS2 and RGS4, inhibit protease-activated receptor 4-mediated signaling by forming a complex with the receptor and Gα in live cells

Fig. 2

Subcellular localization of RGS proteins in the presence of PAR4 and Gα proteins. a-e 293 T cells were transfected with the indicated combinations of PAR4-Venus (PAR4-V, 1.0 μg, a-e), RGS2-HA (1.0 μg, a), RGS4-HA (1.0 μg, b and d), RGS16-HA (1.0 μg, c and e), GαqEE (1.0 μg, a–c), and Gα12EE (1.0 μg, d–e). After immunofluorescence analysis with antibodies against HA and EE, cells were observed under a confocal microscope. Scale bar: 10 μm. f Pearson correlation coefficient analysis was used for quantifying colocalization between PAR4-Venus and RGS-HA proteins in the presence of Gα. ****P < 0.0001, compared to each control. Results are representative of at least three independent experiments

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Cell Communication and Signaling

ISSN: 1478-811X