Fig. 6
Knockdown of miR-153 attenuates the decline in hippocampal presynaptic plasticity in 2VO rats. a Lenti-AMO-153 injection inhibited miR-153 expression in the hippocampi of 2VO rats (PLevene = 0.58, one-way ANOVA: F = 10.301, P = 0.0001). n = 6. b Lenti-AMO-153 attenuated the repression of fEPSP recorded in 2VO rats. χ2Mauchly = 549.258, P < 0.0001; Ftotal (14, 309) = 11.229, P < 0.0001. c Bar graph showing the fold changes in the fEPSP amplitude under 60 μA current stimulation (PLevene = 0.598, one-way ANOVA: F = 8.114, P = 0.002). n = 10. d Lenti-AMO-153 reversed the increase in the PPR in 2VO rats. Right insertion: sample of PPR fEPSP traces. χ2Mauchly = 596.166, P < 0.0001; F(18, 505) = 20.561, P < 0.0001. n = 10 for the 2VO group, n = 9 for the lenti-NCgroup, n = 8 for the lenti-AMO-153 group. e Lenti-AMO-153 prevented the increase in the PPR in 2VO rats (PLevene = 0.001, one-way ANOVA: F = 31.707, P < 0.0001). f-i Lenti-AMO-153 derepressed the protein levels of SNAP-25 (PLevene = 0.324, one-way ANOVA: F = 15.658, P < 0.0001), VAMP-2 (PLevene = 0.752, one-way ANOVA: F = 5.288, P = 0.01), syntaxin-1A (PLevene = 0.817, one-way ANOVA: F = 9.248, P < 0.0001) and Syt1 (PLevene = 0.246, one-way ANOVA: F = 6.3.60, P = 0.003) in hippocampal synaptosomes in 2VO rats. Fisher’s PLSD test was used for the post hoc analyses of the two-group comparisons. n = 6, *P < 0.05 vs Lenti-NC; #P < 0.05 vs 2VO