Fig. 3
MiR-153 regulates the expression of vesicle-related proteins. aMiR-153 levels after transfection with miR-153 mimics and AMO-153 assessed by qRT-PCR. PLevene = 0.060, one way ANOVA: F = 63.818, P < 0.0001; Fisher’s PLSD test was used for post hoc analyses of two-group comparisons. n = 3 batches of neurons per group. b-eMiR-153 downregulates the expression of SNAP-25 (PLevene = 0.411, one-way ANOVA: F = 12.556, P = 0.002), VAMP-2 (PLevene = 0.180, one-way ANOVA: F = 9.350, P = 0.005), syntaxin-1A (PLevene = 0.669, one-way ANOVA: F = 18.496, P = 0.001) and Syt1 (PLevene = 0.200, one-way ANOVA: F = 10.376, P = 0.001) in primary cultured NRNs, and this downregulation was blocked by AMO-153. n = 6 batches of neurons per group. f MiR-153 levels after ODNs transfection assessed by qRT-PCR. n = 3 batches of neurons per group (P < 0.0001). ODN, oligodeoxynucleotides. g–j Gene-specific Snap25-ODN (PLevene = 0.689, one-way ANOVA: F = 13.142, P = 0.002), Vamp2-ODN (PLevene = 0.365, one-way ANOVA: F = 8.235, P = 0.008), Stx1a-ODN (PLevene = 0.124, one-way ANOVA: F = 9.975, P = 0.004) and Syt1-ODN (PLevene = 0.590, one-way ANOVA: F = 23.451, P < 0.0001) reversed the miR-153-mediated repression of the respective proteins in NRNs. n = 4 batches of neurons per group. Fisher’s PLSD test was used for the post hoc analyses of the two-group comparisons. *P < 0.05 vs NC; #P < 0.05 vs miR-153