Fig. 6

OPN promotes HCC glycolysis by modulating αvβ3-NF-κB signaling. a Blockade of integrin αvβ3 with Cilengitide inhibits glucose utilization (n = 3), lactate production (n = 3) and ECAR (n = 5) in HCC-LM3 cells. b Western blotting analysis the signaling pathway influenced by OPN. c Glucose utilization and lactate production in Hep3B cells upon treatment with OPN recombinant protein and indicated pathway inhibitors (n = 3). d Effect of OPN on the NF-κB activity in HCC cells (n = 3). e Effect of CA-IKKβ on the glucose uptake and lactate production in OPN-silenced HCC-LM3 cells (n = 3). *P < 0.05 and **P < 0.01; ns: not significant