Fig. 6
CXCL1 is responsible for the elevation of neutrophil infiltration in TRAF1-deficient mice. a. Traf1−/− mice (n = 3) and WT mice (n = 3) were treated with 40 μg of anti-CXCL1 mAb or control IgG via intraperitoneal injection 2 h prior to C. albicans (1 × 107 CFU) infection. The Skin tissue was harvested one day after infection for immunofluorescence staining and PAS staining. b. OCT-embedded back skin sections from Traf1−/− and WT mice pretreated with anti-CXCL1 mAb or IgG were stained by Ly6G antibody (red) and DAPI (blue), and the Ly6G+ cells were quantified by an average of positive cells from 3 objective fields (200×) each mouse. c. OCT-embedded skin sections from Traf1−/− and WT mice treated with anti-CXCL1 mAb or IgG were stained by PAS. Representative micrographs were captured at 50× magnification. Data are shown as mean ± SEM, and were analyzed using the unpaired, two-tailed, Student’s t-test. Values of p below 0.05 represented a statistically significant difference