Fig. 1
TRAF1-deficiency protects mice from C. albicans intradermal infection. a. Traf1−/− mice (n = 8) and WT controls (n = 8) were intradermally infected with C. albicans SC5314 (1 × 107 CFU) and the area of the skin ulcer was measured every other day. b. Paraffin-embedded skin sections from WT and Traf1−/− mice 3 days post-infection were stained by H&E. Representative micrographs were captured at 50× and 100× magnification. Arrows indicate different layers of the skin region. PBS group, the control group; CA group, the C. albicans infection group. c. Traf1−/− mice (n = 8) and WT controls (n = 8) were intradermally infected with C. albicans (1 × 107 CFU). The clinical scores (scale 0–16) were the sum of individual scores graded as 0 (none), 1 (slight), 2 (moderate), 3 (marked) and 4 (very marked) for ulceration, scab, erythema and nodule, which were recorded every other day for a total of 7 days. The observer was blinded to all biopsy specimens. d. Rag1−/−Traf1−/− mice (n = 6) and Rag1−/− controls (n = 6) were intradermally infected with C. albicans (1 × 107 CFU), and the skin ulcer was measured every other day for a total of 7 days. Individual points represent different mice. Data are pooled from two independent experiments and shown as mean ± SEM, and were analyzed using the unpaired, two-tailed, Student’s t-test. Values of p below 0.05 represented a statistically significant difference