Fig. 2

Nck1 deficiency in BM-MSCs promotes expression of PDGF-A and -C, PDGFRα signaling, and proliferation. a Relative Pdgfa and Pdgfc mRNA levels in growing Nck1^+/+ and Nck1^−/− BM-MSCs isolated from week 5 post-weaning mice (n = 4/group). b PDGF-AA (25 ng/mL)-induced pAKT in over-night starved Nck1^+/+ and Nck1^−/− BM-MSCs isolated from week 5 post-weaning mice (representative of 3 independent experiments). c BrdU incorporation and quantification of BrdU positive Nck1^+/+ and Nck1^−/− BM-MSCs isolated from week 5 post-weaning mice (n = 4/group). Data are mean ± SEM. Statistical significance evaluated by unpaired Student’s t-test and is reported as *p ≤ 0.05, ***p ≤ 0.001, and ****p ≤ 0.0001