Fig. 5
From: Chitinase 3 like 1 suppresses the stability and activity of p53 to promote lung tumorigenesis
Effect of the CBD region of Chi3L1 on binding to p53 and its transcriptional activity (a) After the lung cancer cells were transfected with myc-vector, myc-Chi3L1, myc-CBD, myc-CT, and myc-ΔCBD-ΔCT, the cells were immunoprecipitated with anti-Myc antibody and then the immune complexes were then analyzed by Western blotting with anti-p53 antibody (left). Characterization of the structures in Chi3L1 that bind to p53 is illustrated (right). CBD: chitin binding domain, CT: C-terminal fragment. b The lung cancer cells were transfected with myc-vector, myc-Chi3L1, and myc-Chi3L1 + CBD. After then, the expression of myc-Chi3L1, p53, p21, BAX, cleaved caspase 3, and β-actin were analyzed by Western blotting. c The cells were fixed, permeabilized, and were analyzed by confocal microscopy for detection of intracellular co-localization for myc-Chi3L1 (green) and p53 (red). d p53 luciferase reporter vector that contains two copies of a p53 response element (p53 RE) were co-transfected with myc-vector, myc-Chi3L1, and myc-Chi3L1 + CBD for 24 h, the lysates were harvested and analyzed for luciferase activity. Data shown represent mean ± SEM. *, p < 0.05 compared to myc-vector. #, p < 0.05 compared to myc-Chi3L1