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Table 2 Primer sequences for RT-qPCR

From: 1,25-(OH)2D3/Vitamin D receptor alleviates systemic lupus erythematosus by downregulating Skp2 and upregulating p27

Gene

 

Sequence

Human VDR

Forward Primer

5′-AGCTGGCCCTGGCACTGACTCTGCTCT-3′

Reverse Primer

5′-ATGGAAACACCTTGCTTCTTCTCCCTC-3′

Human Skp2

Forward Primer

5′-GCTGCTAAAGGTCTCTGGTGT-3′

Reverse Primer

5′-AGGCTTAGATTCTGCAACTTG-3′

Human p27

Forward Primer

5′-TAGTCAAAGTGCGAGTGTC-3′

Reverse Primer

5′-TCTGTAGTAGAACTCGGGCAA-3′

Human β-actin

Forward Primer

5′-CATTGCCGACAGGATGCA-3′

Reverse Primer

5′-CATCTGCTGGAAGGTGGACAG-3′

Mouse Skp2

Forward Primer

5′-ATGTGACTGGTCGGTTGC-3′

Reverse Primer

5′-TCGATAGGTCCATGTGCT-3′

Mouse p27

Forward Primer

5′-GCCGAGATATGGAAGAAGCGA-3′

Reverse Primer

5′-AAGAATCTCTGCCCGCAGGTCT-3′

Mouse β-actin

Forward Primer

5′-GCAGTTGGTTGGAGCAA-3′

Reverse Primer

5′-ATGCCGTGGATACTTGGA-3′

  1. Notes: RT-qPCR reverse transcription quantitative polymerase chain reaction, VDR vitamin D receptor, Skp2 S-phase kinase-associated protein 2