Table 1 Bacterial strains and plasmids

DH5α

supE44 ∆lacU169 (φ80 lacZ∆M1) hsdR17 endA1 gyrA96 thi-1 relA1

[35]

E. coli BL21DE3

F⁻ ompT hsdS_B (r_B ^–m_B⁻) gal dcm

Novagen

H. pylori 26695

Wild-type strain

[36]

H. pylori J99

Wild-type strain

[37]

H. pylori G27

Wild-type strain

[38]

H. pylori 7.13

Wild-type strain

[39]

H. pylori N6

Wild-type strain

[40]

H. pylori N6∆htrA

H. pylori N6 secAR837K ∆htrA, Kan^R

[28]

H. pylori N6 ∆htrA/htrA_N6

H. pylori N6 secAR837K ∆htrA/htrA_N6, Cm^R

[28]

H. pylori N6 ∆htrA/htrA_N6 S221A (S/A)

H. pylori N6 secAR837K ∆htrA/htrA_N6 S221A, Cm^R

[28]

pJS17

pQE60, htrA S210A from E.coli with C-terminal His₆-tag, Amp^R

[41]

pUZ3

pET26b, htrAS221A from the H. pylori 26695 strain with C- terminal His₆-tag, Kan^R

[27]