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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Dimerization of MORC2 through its C-terminal coiled-coil domain enhances chromatin dynamics and promotes DNA repair

Fig. 5

The C-terminal coiled-coil domain of MORC2 is required for its dimer formation. a HEK293T cells were transfected with either Flag-MORC2 or HA-MORC2 ΔC82. After 48 h of transfection, immunofluorescent staining was carried out using an anti-Flag or an anti-HA antibody. Nuclei were counterstained with DAPI. b HEK293T cells were transfected with HA-MORC2 and HA-MORC2 ∆C82. After 48 h of transfection, total cellular lysates were subjected to cross-linking assay, followed by immunoblotting with an anti-Flag antibody. c HEK293T cells were transfected with HA-MORC2, HA-MORC2 ∆C82 alone or in combination with Flag-MORC2. After 48 h of transfection, total cellular lysates were subjected to IP analysis with an anti-Flag or an anti-HA antibody, followed by immunoblotting with the indicated antibodies

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Cell Communication and Signaling

ISSN: 1478-811X