Fig. 7
From: Comparative phosphorylation map of Dishevelled 3 links phospho-signatures to biological outputs
Comparison of individual methods and their validation by phospho-specific antibodies. a-d. Comparison of methods. Existing phosphorylation-specific antibodies were used to visualize the level of phosphorylation of DVL3 with our kinase panel. HEK293 cells were transfected by the indicated combination of plasmids and analyzed by Western Blotting. Reactivity of individual phosphoantibodies against DVL3 phosphorylated by individual kinases is shown. Western blots are quantified using ImageJ software as absolute values for peak areas of corresponding bands and the intensities were normalized to the control. Mean intensities of the phosphorylated peptides obtained by MS/MS via pipelines #1 and #2 are shown in the shades of grey. Numbers indicate decadic logarithm of the mean peptide intensity. “Not detected” (ND) indicated in white corresponds to the signals below 1 × 106, i.e. 6.0. a. anti-pS192 (this study), b. anti-pS280, c. anti-pS643, d. anti-pS697