Table 1 Sequences of primers for PCR amplification of targeting sites
From: Targeting the orphan nuclear receptor NR2F6 in T cells primes tumors for immune checkpoint therapy
Primer | Primer Sequences (5′ to 3′) | Amplicon (bp) |
|---|---|---|
CD44.03.F | TGCCTCTGTCCGTCTTCATC | 561 |
CD44.03.R | CCTGTGCACTCTCCATCCTC | |
CD44.01.02.F | GAAGTTGCTGGGGTGGGAAT | 545 |
CD44.01.02.R | ACACGGATGGGTTTGCAAAA | |
CD44.04.F | GCAGAGTCTGTCTTAGGGTGT | 513 |
CD44.04.R | GCACTGGTTAAAGGAGGGGT | |
CD69.01.F | CCCCTCCCAGTGTTTTCTGT | 555 |
CD69.01.R | AGTGCGTTGATGAGGCTTCA | |
CD69.02–04.F | TGTTCATGCCCCATCCTGTC | 505 |
CD69.02–04.R | CATACAGTGCTCCCTGCCTC | |
Nr2f6.01.05.F | CTCTATCGCCTGGACCTCTG | 934 |
Nr2f6.01.05.F | AGGAAGTTGCCGCAAAACT | 580 |
Nr2f6.01.05.R | GCACGCATGTATCACCAACT | |
Nr2f6.02.F | AGGCCAGAGACAGAAGCTGA | 764 |
Nr2f6.02.F | GTGTTTAGGTGGGGCTCTGA | |
Nr2f6.03.04.F | TAGCAATGCCCCTCCTATTG | 745 |
Nr2f6.03.04.R | GCCTTTCCAGATACCCATGA |