Table 1 The table of primers and sequence information
Transcript Name | Primer Sequence (5′-3′) |
|---|---|
ZNF81 | Forward primer: TGATACAGAAGACTTGAGATT Reverse primer: TCACAAAGTATTCACATTACC |
Exonic LINC00478 | Forward primer: TCAAGTTCAGTGTTTGGTTAA Reverse primer: GGCAGAATCGTGAATAGC |
Intronic LINC00478 | Forward primer: AACAGGTCACAATGGTGGAATG Reverse primer: TGAAGCAACTGAAGATCCACAA |
Beta-2-microglobulin | Forward primer: CGGGCATTCCTGAAGCTGA Reverse primer: TGGAGTACGCTGGATAGCCT |
Beta-actin | Forward primer: GTGCGCCGTTCCGAAAGT Reverse primer: ATCATCCATGGTGAGCTGGCG |
Synthetic RNA Spike-in (100 bp from Isopenicillin N-CoA synthetase) | Spike-in Forward primer: TACTGCATCCCGCTCTAC Spike-in Reverse primer: CGCTCATCAAGTCGTTCA Spike-in RNA sequence: UUGGGCAGAAACCGGGCCCCAACGGUGACCGCACCUACU ACUGCAUCCCGCUCUACCACGGAACGGGGGGCAUCGCGGCCAUGAACGACUUGAUGAGCGG |