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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Specific trophoblast transcripts transferred by extracellular vesicles affect gene expression in endometrial epithelial cells and may have a role in embryo-maternal crosstalk

Fig. 2

Visualization of 5-ethynyl uridine (EU)-labelled RNA in trophoblast spheroids and endometrial cells. a RNA in trophoblast spheroids were labelled with 5-ethynyl uridine (EU) and stained with Alexa azide. Green florescence is evidence of successful labelling. a1 Unlabelled spheroids (negative control) did not show fluorescent signal. b Endometrial cells were stained with Alexa azide after 24 h incubation with labelled spheroids to visualize the transferred transcripts. Green dots in endometrial cells indicate labelled RNA transfer. b1 Endometrial cells co-incubated with unlabelled spheroids were used as negative controls. Negative control did not exhibit any specific fluorescent signal. c, c1 3-dimentional confocal scanning of endometrial cells with cytoplasmic EU labelled RNA with and without cell tracker dye. Scale bar 4 μm

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Cell Communication and Signaling

ISSN: 1478-811X