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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Linoleic acid improves assembly of the CII subunit and CIII2/CIV complex of the mitochondrial oxidative phosphorylation system in heart failure

Fig. 3

Cardiolipin content and mitochondrial respiration were decreased in cardiomyocytes by siRNA-mediated blockade of cardiolipin synthase 1 (CRLS1). Assays were started at 72 h after the transfection. a. Amount of CRLS1 in isolated mitochondria from cardiomyocytes treated with irrelevant siRNA (Irr) (n = 6) or siCRLS1 (n = 6) (left). Total protein levels shown by Ponceau staining were used as the internal controls (right). The sizes of the molecular weight markers are indicated on the left in kDa. b. Cardiolipin content in isolated mitochondria from cardiomyocytes treated with an irrelevant siRNA (Irr, n = 5) or siCRLS1 (n = 5). C. Complex I-linked respiration, complex I + II-linked respiration, and complex II-linked respiration in isolated mitochondria from cardiomyocytes treated with control (n = 4) and siCRLS1 (n = 4). Data are shown as the mean ± SE. *p < 0.05 vs Irr

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Cell Communication and Signaling

ISSN: 1478-811X