Fig. 3
From: Induction of LEF1 by MYC activates the WNT pathway and maintains cell proliferation
LEF1 increases nuclear retention of β-catenin in MYC-overexpressing cells. a Rat1 myc−/− fibroblasts with or without MYC expression were fractionated into cytosolic and nuclear fractions, and the samples were subjected to Western blotting with the indicated antibodies. b Rat1 fibroblasts were incubated with 20 μg/mL Leptomycin B for 3 h before fractionation and Western blotting with the indicated antibodies. c-e Rat1 fibroblasts (c), DLD1 cells (d) and HCEC cells with APC truncation mutation (e) were transfected with either control or LEF1 siRNA, and then fractionated for Western blotting with the indicated antibodies. f Model of activation of β-catenin by LEF1 in MYC-expressing cells