Fig. 7
High CBFβ expression correlates with CDK11 expression and contributes to metastasis and reduced overall survival in patients. a Representative images of different immunohistochemical staining intensities of CBFβ and CDK11 staining are shown in osteosarcoma tissues. CBFΒ and CDK11 immunoreactivity was found mostly in the nucleus of tumor cells. The percentage of cells showing positive nuclear staining for CBFβ or CDK11 was calculated by reviewing the entire spot (original magnification, 200×). b The correlation coefficient (R2) between CBFβ expression and CDK11 expression was derived by GraphPad PRISM 5 (correlation coefficient R2 = 0.7729, P < 0.001). c Kaplan-Meier survival curve of patients with osteosarcoma were subgrouped as either CDK11 low staining (CDK11 staining ≤3) or high staining (CDK11 staining ≥4). d Kaplan-Meier survival curve of patients with osteosarcoma were subgrouped as either CBFβ low staining (CBFΒ staining ≤3) or high staining (CBFβ staining ≥4). e Distribution of CDK11 staining scores from the same patients with metastatic and primary specimens. f Distribution of CBFβ staining scores from the same patients with metastatic and primary specimens