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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Fibroblast-derived CXCL12 regulates PTEN expression and is associated with the proliferation and invasion of colon cancer cells via PI3k/Akt signaling

Fig. 6

The different concentration of CXCL12 activates phosphorylation of PI3K in HT-29, Colo320, CaCo-2 and PTEN siRNA transfected HT-29 cells. (a) HT-29, Colo320, CaCo-2 cells were treated with 1 ng/ml, 10 ng/ml and100ng/ml of CXCL12, and incubated for 15 min. The cells were collected from each time point, lysed by lysis buffer, and immunoblotted with a phospho-PI3K antibody as described in Materials and Methods. Detection of total PI3K levels served as a loading control. (b) The different concentration of CXCL12 affects phosphorylation of PI3K and total PI3K in the PTEN siRNA transfected and control siRNA HT-29 cells. β-actin served as a loading control

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