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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: 4.1B suppresses cancer cell proliferation by binding to EGFR P13 region of intracellular juxtamembrane segment

Fig. 7

4.1B FERM domain binds to EGFR JM segments. a Sketch map of intracellular EGFR design information. GST-tagged three fragments of intracellular EGFR: EGFR fragment with (amino acids fragment 644–955 (EGFR1)) and without (amino acids fragment 956–1186 (EGFR2)) JM fragments and JM fragment deletion the first thirteen amino acids (amino acids fragment 657–955 (EGFR1△aa13)). The black arrow shows EGFR fragment (amino acids fragment 644–955 (EGFR1)) with two or three amino acids mutated randomly: aa13-RRRHIVRKRTLRR, EGFR P13(RRR-AAA)-AAAHIVRKRTLRR, EGFR P13(RKR-AAA)- RRRHIVAAATLRR, EGFR P13(RR-AA)-RRRHIVRRRTLAA. AAA means mutated region. b 4.1B binds to EGFR fragments with the first thirteen amino acids of JM fragment by pull-down assay. c Sketch map of domains of 4.1B and the FERM domain of 4.1B binds to EGFR by pull-down assay. d Conclusions of 4.1B FERM domain interacts with EGFR fragment with the first thirteen key amino acids of JM fragment

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