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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: cAMP- and cGMP-elevating agents inhibit GPIbα-mediated aggregation but not GPIbα-stimulated Syk activation in human platelets

Fig. 2

EB-induced Syk tyrosine phosphorylation is dependent on src family kinases (SFKs). Human washed platelets (WP) were pre-incubated for 5 min with vehicle control (DMSO), the SFK inhibitor, PP2 (10 μM) or with its inactive analogue, PP3 (10 μM) prior to stimulation with EB. a, d Syk phosphorylation at Y525/526 and Y352 was analyzed at early time points (0, 15, 60 and 120 s). b, c, e, f Quantitative analysis of Syk Y525/526 and Y352 phosphorylation compared to total Syk. Data are means ± S.D. from at least 3 independent experiments with platelets from at least 3 healthy donors; ns: not significant, ***p < 0.001, ****p < 0.0001

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