Fig. 4
Autophosphorylation of KITN822K and KITV560G occurs preferentially on the Golgi in leukemia cells. a & b Kasumi-1 (a) or HMC-1.1 cells (b) were immunostained for KIT (green), phospho-KIT Y703 (pKITY703, red or green) together with GM130 (Golgi marker, blue), PDI (protein disulfide isomerase, ER marker, red), TFR (endosome marker, red), or LAMP1 (lysosome marker, red). Insets show magnified images. Bars, 10 μm. c Pearson’s R correlation coefficients were calculated by analyzing the intensity of pKITY703 vs. organelle markers. Results are means ± s.d. (n = 12~21). ***P < 0.001. Note that pKITY703 was colocalized with GM130 rather than with PDI, TFR, or LAMP1 both in Kasumi-1 and HMC-1.1 cells