Fig. 6

Effects of β-catenin signaling inhibitor on lipid accumulation in hepatic cells. a Ubiquitination of β-catenin following immunoprecipitation (IP) with an anti-HA antibody and immunoblot analysis for β-catenin, and IP of an anti-Flag antibody and immunoblot analysis for HA-ubiquitin. Flag-β-catenin-overexpressing HepG2 cells were transfected with HA-ubiquitin and/or Myc-parkin. b Hepatic lipid accumulation in primary hepatic cells from WT or parkin KO mice pretreated with IWR-1 (40 μM) and then treated with ethanol (100 mM) (Scale bars, 50 μm). c Expression of genes involved in lipid metabolism in primary hepatic cells from WT or parkin KO mice pretreated with IWR-1 (40 μM) and then treated with ethanol (100 mM). Values are expressed as the mean ± SD of three different experiments conducted in triplicate. ^*P < 0.05, pretreated with IWR-1 versus without; ^#P < 0.05, WT versus KO