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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Inhibition of T cell immunoglobulin and mucin-1 (TIM-1) protects against cerebral ischemia-reperfusion injury

Fig. 1

TIM-1 expression is upregulated in MCAO. a TIM-1 mRNA expression was detected by quantitative reverse-transcription PCR (qRT-PCR) at 24 h and 48 h after MCAO. *p < 0.05; **p < 0.01 vs Black. b-c Western blot detection of the level of TIM-1 protein expression 24 h and 48 h after MCAO. *p < 0.05; **p < 0.01; ***p < 0.001 vs Black. d Flow cytometry was used to measure the level of TIM-1 expression in the PBMCs. e-f TIM-1 protein expression and miRNA were examined by Western blot and qRT-PCR following treatment with or without the CD3 monoclonal antibody-stimulated T cells cultured in vitro. ***p < 0 .001 vs Control. g The level of TIM-1 expression was detected by flow cytometry following treatment with or without in vitro CD3 monoclonal antibody stimulation

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