Fig. 6

9F7-F11 activates the extrinsic apoptotic pathway through FAS and DR5 upregulation, TRAIL expression induction and DcR2 downregulation. a BxPC3, MDA-MB-468, and DU145 cells were treated with 9F7-F11 for various times. FAS, DR5, mTRAIL and DcR2 expression were detected by western blotting. b BxPC3 cells were incubated with 9F7-F11. After cells lysis at different time points, total protein extracts (2 mg) were co-immunoprecipitated with the human anti-DR5 polyclonal antibody D4E7 (Cell Signaling Technology) or with human anti-IgG as control. The presence of DISC components (caspase-8, FADD and DR5) was assessed in immunoprecipitates and whole cell lysates (WCL) by western blotting. Protein level was measured with the ImageJ software and indicated as signal intensity (SI), relative to untreated control (SI = 1.0 ± .0). Significant increase or decrease of the densitometry, compared to control, is indicated in bold. β-tubulin was evaluated as loading control