Fig. 4

Estrogen-upregulated c-MYC is a crucial GLS promoter. a, b Based on TCGA database (https://tcga-data.nci.nih.gov), survival time of UEC patients were analyzed referring to c-MYC (a) or n-MYC (b) expression. c, d GLS levels of c-MYC (c) or n-MYC (d) overexpressed Ishikawa and KLE cells were detected by qRT-PCR. e Ishikawa and KLE cells were treated with estrogen (10 nM) and/or Mycro3 (50 μM) for 48 h and then GLS levels were tested by qRT-PCR. f Protein levels of GLS in Ishikawa and KLE cells were detected by western blotting after treatment with estrogen and/or Mycro3 for 24 h. The data are expressed as the mean ± SD. *P < 0.05; *** P < 0.005; ****P < 0.001 and ns means no statistical significance. OE-c-MYC: c-MYC overexpressed cells; OE-n-MYC: n-MYC overexpressed cells