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Fig. 10 | Cell Communication and Signaling

Fig. 10

From: Novel interactions between ERα-36 and STAT3 mediate breast cancer cell migration

Fig. 10

ERα and JAK2-STAT3 signaling cross-talking human breast cancer cell. a and b, Transwell assay was used to detect the migration-stimulating effects of TAM and AG490 or E2 and IL-6 in MDA-MB-231 cells. The number of cells migrated to the lower side of the transwell chambers was counted and photographed in five fields (the upper, the lower, the left, the right, and the middle) of three independent experiments, and the fold migration was calculated. (The negative control (DMSO) of Fig. 10a and the negative control (DMSO/PBS) of Fig. 10b) (*P < 0.05, **P < 0.01.n = 3). c and d, The effect of overexpressed TAM and AG490 or E2 and IL-6 on MDA-MB-231 migration of was detected by Wound-healing assay. E, MDA-MB-231, MCF-7 and MCF-10A cell lysates were immunoprecipitated with an anti-ERα antibody and probed with an anti-STAT3 antibody. f and g, Effects of IL-6 on ERE transcription activity (f) and effects of IL-6 on MMP2 and MMP9 transcription activity (g) were investigated in MDA-MB-231 cells. The luciferase activity was expressed as a percentage relative to the control was used. All luciferase assays were performed in duplicates. (*P < 0.05, **P < 0.01.n = 3)

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