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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Caffeic acid phenethyl ester suppresses androgen receptor signaling and stability via inhibition of phosphorylation on Ser81 and Ser213

Fig. 4

CAPE treatment suppressed AR protein level by accelerating the degradation of AR. Protein abundance of AR in nucleus and cytoplasm of LNCaP 104-S (a) and LNCaP 104-R1 (b) cells being treated with or without DHT and increasing concentration of CAPE for 48 h was determined by Western-blotting. GAPDH and lamin A/C were used as loading control for cytoplasmic and nuclear extract, respectively. LNCaP 104-S and LNCaP 104-R1 (c) cells were treated with 10 μg/ml cycloheximade (CHX) plus 40 μM CAPE or/and 1 nM DHT for 4, 8, 24, and 48 h. AR protein level was determined by Western blotting

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Cell Communication and Signaling

ISSN: 1478-811X