Fig. 5
From: WDR76 degrades RAS and suppresses cancer stem cell activation in colorectal cancer
WDR76 induces proteasomal degradation of wild-type and oncogenic K-RAS in CRC cells. a-b Western blots of extracts from D-WT and D-MT cells stably expressing GFP-Control or GFP-WDR76 a and shControl-GFP or shWDR76-GFP b using the indicated antibodies. c Western blots of extracts from D-WT and D-MT cells stably expressing GFP-Control or GFP-WDR76 with or without MG-132 treatment (20 μM, 4 h) using the indicated antibodies. d-e Immunoprecipitation d and ubiquitination e of K-RAS in ALLN-treated (25 μg/mL, 12 h) D-WT and D-MT cells stably expressing GFP-Control or GFP-WDR76 with immunoblotting against the indicated antibodies. f Relative mRNA levels of the indicated genes from D-WT and D-MT cells stably expressing either GFP-Control, GFP-WDR76, shControl-GFP, or shWDR76-GFP quantified by RT-qPCR. n.s.: not significant