Fig. 3

EGF stimulation increases leucine uptake. a and b, leucine uptake was examined 5, 15 or 30 min after EGF stimulation of LNCaP (a) or PC-3 cells (b). Two-tailed student’s t-test was performed. Data are the mean ± SEM, n = 4. c and d, the phosphorylation of Akt was examined 5, 15 or 30 min after EGF stimulation of LNCaP (c) or PC-3 cells (d). GAPDH was used as loading control. All western blot images are representative of three independent experiments