Fig. 6

Din enhanced the tumor inhibitory effect of regorafenib in vivo. Male BALB/c athymic knockout mice were subcutaneously inoculated with Huh-7 cells. They were then administered regorafenib (20 mg/kg/d), with and without Din (30 mg/kg, every other day). a Tumor size difference (for each group, n = 5). b Representative tumors from each group. c The body weight of mice in each treatment groups. d The expression of cleaved caspase 3/8 and Mcl-1 in the tumors from the different groups. e Changes in tumor cell apoptosis (TUNEL assay) after drug treatment. The western blots and immunostaining were repeated for 3 times, and representative data were shown. **, P < 0.05