Fig. 5

Din mediated the downregulation of Mcl-1 by suppressing transcription of STAT3. a The levels of Mcl-1 mRNA in Huh7 cells after treatment with 50 nM Din at the indicated time points. b Mcl-1 protein levels in Huh7 cells treated with 50 nM Din with or without pretreatment of 1 μg/ml CHX. c The expression of p-STAT3, and total STAT3 in Huh7 cells treated with 50 nM Din at different time points. d The binding of STAT3 to Mcl-1 proteins in Huh7 cells and treated with 50 nM Din for 24 h. e The luciferase reporter activity of pGL-Mcl-1 reporter in Huh7 cells transfected with control or STAT3 plasmids, followed by 50 nM Din treatment. f Expression of STAT3, cleaved caspase-3, and Mcl-1 in Huh7 cells transfected with the control or STAT3 plasmid and treated with 50 nM Din. g Hoechst 33258 staining for apoptosis of Huh7 cells transfected with the control or STAT3 plasmid and treated with 50 nM Din. The western blots were repeated for 3 times, and representative data were shown. N = 3 for a, e, g. **, P < 0.05