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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Characterization of CD44 intracellular domain interaction with RUNX2 in PC3 human prostate cancer cells

Fig. 6

The effect of RUNX2 overexpression on the expression of metastasis-related genes. a-e. The real-time PCR analysis of OCT4, SOX2, CD44, OPN, and MMP9 expression in PC3, PC3 cells transfected with RUNX2, and LNCaP cells. f. An equal amount of protein lysates (30 μg) made from PC3 (lane 1) and PC3 cells transfected with RUNX2 (lane 2) were immunoblotted with Ezrin, OPN, RUNX2, SOX2, MMP9 antibodies to detect total cellular levels of the respective proteins. GAPDH was used as a loading control for real-time PCR and IB analysis (A-G). The results represent one of three separate experiments performed with the same results

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