Fig. 1
From: NR6A1 regulates lipid metabolism through mammalian target of rapamycin complex 1 in HepG2 cells
The effect of NR6A1 knockdown on insulin-induced proliferation and migration in HepG2 cells. HepG2 cells were transfected with scrambled or NR6A1 siRNA for 48 h. Western blotting (a) and qPCR assay (b) were used to confirm NR6A1 knockdown efficiency. Representative blots were shown. n = 3. (c) The insulin (10 nM) stimulated migration of control and NR6A1 knockdown cells was analyzed by a typical scratch wound healing assay. Representative images were shown. Bar = 200 μm. n = 3. (d) The ratio of wound closure compared with control. n = 3. *P < 0.05. (E) Control and NR6A1 knockdown HepG2 cells were treated with vehicle or insulin (10 nM) for 24 h. Cell proliferation was measured using CCK-8 proliferation kit. Results were obtained from 3 independent experiments. *P < 0.05