Fig. 7
From: ErbB4 promotes malignant peripheral nerve sheath tumor pathogenesis via Ras-independent mechanisms
Erbb4 increases the phosphorylation of a number of other cytoplasmic kinases, independent of Ras activation. a Graphical representation of quantified kinase arrays comparing the levels of baseline phosphorylation to NRG1 stimulated phosphorylation in Erbb4-expressing UBI MPNST cells. The graph includes the subset of kinases whose phosphorylation was altered following NRG1β stimulation. b Graphical representation of quantified kinase arrays comparing the levels of baseline phosphorylation of control Erbb4- intact cells to NRG1 stimulated ErbB4-intact and Erbb4-null UBI MPNST cells to determine NRG1 dependent and ErbB4 dependent kinases. c) Quantification of a subset of the non-responsive kinases ErbB4-intact compared to ErbB4-ablated to identify targets positively regulated by ErbB4 independent of stimulation. d) Quantification of a subset of the non-responsive kinases ErbB4-intact compared to ErbB4-ablated to identify compensatory targets resulting from ErbB4-ablation independent of stimulation. Quantification of the kinases differentially phosphorylated was quantified per the manufacturer’s protocol using ImageJ