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Fig. 9 | Cell Communication and Signaling

Fig. 9

From: Minocycline impairs TNF-α-induced cell fusion of M13SV1-Cre cells with MDA-MB-435-pFDR1 cells by suppressing NF-κB transcriptional activity and its induction of target-gene expression of fusion-relevant factors

Fig. 9

Minocycline inhibits TNF-α -induced activation of NF-κB-p65 gene expression in a dose-dependent manner. M13SV1-Cre cells were transiently transfected with an NF-κB promoter in a luciferase reporter vector and pre-incubated with different concentrations of minocycline (50 μM, 100 μM) or PDTC (50 μM) for 24 h, followed by TNF-α (100 ng/ml) stimulation for 2 h. Shown are the means ± STD from at least three independent experiments for which the luciferase activity of transfected cells was calculated in relation to nontransfected M13SV1-Cre cells, for which the value was set to 1. Statistical analysis was conducted with an ANOVA F-test and Scheffé post hoc test: * = p < 0.05; ** = p < 0.01; *** = p < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X