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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: Minocycline impairs TNF-α-induced cell fusion of M13SV1-Cre cells with MDA-MB-435-pFDR1 cells by suppressing NF-κB transcriptional activity and its induction of target-gene expression of fusion-relevant factors

Fig. 7

Minocycline inhibits NF-kB-p65 nuclear translocation/ activation in M13SV1-Cre cells but not in MDA-MB-435-pFDR1 cells. a Cytoplasmic and nuclear cell lysate extracts of M13SV1-Cre and MDA-MB-435-pFDR1 cells under control conditions, TNF-α (100 ng/ml) induction, minocycline treatment (100 μM) or minocycline + TNF-α exposure were collected and analysed by western blotting. b PDTC (50 μM) was used as an additional control to block NK-κB. Relative NF-κB p65 protein and phosphorylated protein levels in the cytoplasm and in the nucleus were normalised to the levels of β-actin and histone H3, respectively, and in relation to control, which was set to 1. Representative western blot data of at least three independent experiments are shown. Statistical analysis: ANOVA F-test and Scheffé post hoc test: * = p < 0.05; ** = p < 0.01; *** = p < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X