Fig. 9
From: YAP promotes neural crest emigration through interactions with BMP and Wnt activities
BMP and Wnt mediate YAP-dependent stimulation of NC EMT. (a-d’) Electroporation for 16 h of control GFP (a,a’), YAP (b,b′), Noggin (c,c′), or Noggin+YAP (d,d’). YAP stimulates NC EMT and Noggin inhibits the process. YAP is unable to rescue cell emigration in the absence of BMP signaling (Noggin+YAP). (e) Data quantification.**p < 0.001 for YAP/control; ***p < 0.0001 for Noggin/control and Noggin+YAP/YAP, and ***p < 0.0002 for Noggin+YAP/control. (f-i′) Electroporation for 16 h of control GFP (f,f′), YAP (g,g’), Xdd1 (h,h′), or Xdd1 + YAP (i,i′). YAP stimulates and Xdd1 inhibits NC EMT, respectively. YAP mildly rescues cell emigration in the absence of Wnt signaling when compared to Xdd1 alone (Xdd1 + YAP). (e) Data quantification. **p < 0.0006 for YAP/control; ***p < 0.0001 for Xdd1/control; ***p < 0.0003 for Xdd1 + YAP/control, ***p < 0.0001 for Xdd1 + YAP/YAP and *p < 0.04 for Xdd1 + YAP/Xdd1. (a’-d’ and f′-i′) Nuclei were stained with Hoechst (grey). Bars = 50 μM