Fig. 5
From: YAP promotes neural crest emigration through interactions with BMP and Wnt activities
Gain of YAP function inhibits neuronal differentiation in DRG and stimulates the proportion of EdnrB2-positive melanocytes in the subectodermal pathway. (a-d”) Transverse sections of DRG in 4 day-old embryos electroporated two days earlier with control GFP (a, c-c″) or with YAP (d-d”). Note the even distribution of transfected cells throughout the DRG in A and its preferential peripheral distribution in B. (c-d”) Co-labeling of GFP and Islet-1. Arrows in c-c″ depict electroporated cells co-expressing GFP and Islet-1; arrowheads in D-D” point to YAP-GFP+/Islet-1-negative progenitors. (e) Quantification of the percentage of Islet+/GFP+ neurons over total GFP+ cells (***p < 0.0001). (f-i″) Transverse sections of 4 day-old embryos electroporated two days earlier with control GFP (f, h-h″) or with YAP (g, i-i″). (h-i″) represent co-labeling of GFP with in situ hybridization for Ednrb2. Arrows depict electroporated cells co-expressing GFP (green) and Ednrb2 (blue). (j) Quantification of the percentage of GFP+/Ednrb2+ melanocytes in the lateral pathway over total Ednrb2+ cells (*p < 0.001). Bars = 50 μM