Fig. 4
From: YAP promotes neural crest emigration through interactions with BMP and Wnt activities
YAP stimulates G1/S transition, mitosis and survival of premigratory NC cells. (a-b″) Brdu immunostaining of S-phase nuclei (red) of scrambled RNA (a-a”) and shYAP (b′-b″)-electroporated embryos (green). Arrows in a-a” depict GFP+/Brdu+ nuclei and arrowheads in b-b″ point to GFP+/Brdu-negative cells. (c-d”) Immunostaining for mitotic figures with a phospho-histone H3 antibody (green) of scrambled RNA (c-c″) and shYAP (d-d”)-electroporated embryos (red). (e-f′) Visualization of apoptotic nuclei by Tunel (red) in scrambled RNA (e,e’) and shYAP electroporated embryos (f,f′) (green). Nuclei are visualized with Hoechst. (g) Data quantification restricted to electroporated cells in the dorsal 20% of the neural tube (NT). Upper graph represents incorporation of Brdu into nuclei (*p < 0.001); middle graph is the quantification of mitotic figures (**p < 0.003), and lower panel represents apoptotic nuclei (**p < 0.0007). Bar = 50 μM