Fig. 2

a Scatter plot displaying SILAC ratios of setups SR (x-axis) and I + 5′S (y-axis). S/T-P motifs: triangles. Other motifs: circles. Ratios are log₂-transformed. Similar analyses were made with setups I + 0′S and I + 10′S, respectively (not shown). b Results from gene ontology (GO) enrichment for three sets of Hog1-dependent phosphorylation sites derived from MQ, PD, and both search engines. GO-terms were filtered to have at least one q-value ≤0.1 in either set, allowing hierarchical levels ≥2 and solely “biological processes” as a GO category. The bubble size corresponds to the number of proteins associated with a given term; the color corresponds to the fold enrichment. The GO-terms were sorted according to the average fold-enrichment (side bar plot). On the right-hand side the PD/MQ-ratio between the respective enrichments are shown as a bar plot. In case the ratio is ≥1 (signified by red dashed line), the enrichment of the corresponding term is higher in PD vs. MQ (yellow coloring), and vice versa (blue coloring). c Venn diagram showing percentage and total number of stress- and Hog1-induced S/T-P motifs. Light blue: MQ, yellow: PD, green: overlap. d Putative Hog1-target proteins identified via genuine S/T-P (left) or S/T-S/T-P (right) motifs. Color-coding similar to (c). Filled arrowheads: known Hog1-target proteins in MQ-derived dataset. Open arrowheads: candidates that did not qualify as Hog1-substrates in [4] due to lack of overlap between experimental setups. *: alternative phosphorylation sites found with PD or MQ