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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Targeting of the AXL receptor tyrosine kinase by small molecule inhibitor leads to AXL cell surface accumulation by impairing the ubiquitin-dependent receptor degradation

Fig. 5

BMS777607 (BMS) impaired GAS6-dependent internalization of AXL. Flow cytometry analysis of Hs578T cells after stimulation of AXL by 250 ng/ml exogenous GAS6 for 30 min and 2 hours in combination with BMS treatment. Two different extracellularly binding anti-AXL antibodies (Ab 154 for a, and Ab 259/2 for b-f) were used. Membrane permeabilisation was implemented to distinguish between the membranous and total AXL fraction. Representative histogramms of flow cytometry analysis are displayed for Ab 259/2 in the non-permebilized membranious AXL fraction of Hs578T cells (c-d) and membrane permebilized total AXL protein fraction (e-f). Histogramm C and E represent the DMSO treated Hs578T control cells and D and F show histograms of 0.5 μM BMS treated Hs578T cells. Mean values and SEM of three independent experiments are shown. Differences with *P < .05, **P < .01, ***P < .001 and ****P < .0001

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