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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Elevated Kallikrein-binding protein in diabetes impairs wound healing through inducing macrophage M1 polarization

Fig. 6

KBP promotes the M1 polarization of macrophages via activating the Notch signalling pathway. a The expression of Notch1 in the wounds of WT and KBP-TG mice. Scale bar = 50 μm. b The mRNA expression of Notch1, Notch2 and other transcription factors (RBP-Jκ) or target genes associated with Notch signalling in RAW264.7 cells. c DAPT inhibits the effect of KBP on the activation of Notch signalling in RAW264.7 cells. d Western blot to detect Notch1, Hes1, iNOS and ARG1 following the treatment of RAW264.7 cells with KBP and DAPT. e, g The expression of iNOS and ARG1 in RAW264.7 cells following treatment with siHes1 and siRBP-Jκ. (F, H) The expression of iNOS and ARG1 in RAW264.7 cells following treatment with KBP and siHes1 or siRBP-Jκ. Data are presented as the mean ± SD. n = 3; * p < 0.05. Three independent experiments were employed

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