Fig. 3

The expression of CD49f and NOTCH1. a, Heat map analysis of the differentially expressed genes in PRAD patients from TCGA. b, Mean mRNA analysis of NOTCH1 and NOTCH4 in PRAD of TCGA. Significance was assessed using Student’s paired t-test. The scatter dot plot is presented as the median. ***, P < 0.001. c, The correlation of CD49f or ERα and NOTCH1 in normal prostate and PRAD from TCGA (num (Normal) = 52), num (PRAD) = 498)) and GTEx (num (Normal) = 100)). d, IHC staining of CD49f and NOTCH1 in normal prostate tissues and cancer tissues with different Gleason score. Scale bar, 200 μm. e, Flow cytometry analysis of the coexpression of CD49f and NOTCH1 in LNCaP-abl and PC3 treated with either DMSO or 10 nM E2 for 72 h (n = 3). f, Western blot analysis of the indicated proteins in LNCaP-abl or PC3 cells treated with 10 nM E2 treatments for 72 h (n = 3). g, qRT-PCR analysis showing expression changes of the indicated genes in LNCaP-abl treated with either DMSO or 10 nM E2 for 72 h. The data are presented as the means±SD (n = 3). *, P < 0.05 vs. siNC