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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Oridonin protects LPS-induced acute lung injury by modulating Nrf2-mediated oxidative stress and Nrf2-independent NLRP3 and NF-κB pathways

Fig. 6

Effects of Ori on histological changes and inflammatory protein expressions in LPS-induced ALI. a Mice were given an intraperitoneal administration of Ori (20 and 40 mg/kg) 1 h prior to an intranasal administration of LPS. Lungs (n = 5) from each experimental group was processed for histological evaluation at 12 h after LPS challenge: Control, LPS group, Ori (40 mg/kg), LPS + Ori (20 mg/kg), LPS + Ori (40 mg/kg). Representative histological section of the lungs was stained by hematoxylin and eosin (H&E staining, magnification × 200). b and d Protein expressions of NLRP3, ASC, CASPASE-1, IL-1β, TXNIP, TRX-1, P-P65, P65, P-IκBα and IκBα were measured by Western blot analysis. c, e and f Quantification of expressions of previous protein was performed by densitometric analysis and β-actin was acted as an internal control. All results were expressed as the means ± SEM of three independent experiments. *p < 0.05 and **p < 0.01 versus the control group, #p < 0.05 and ##p < 0.01 versus LPS group

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