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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Oridonin protects LPS-induced acute lung injury by modulating Nrf2-mediated oxidative stress and Nrf2-independent NLRP3 and NF-κB pathways

Fig. 4

Ori inhibited LPS-induced inflammatory protein expression in RAW 264.7 cells. Cells were exposed to Ori (2.5, 5 or 10 μM) for 6 h and then treated with LPS (1 μg/ml) and ATP for 1 h and 40 min, respectively. d Protein expressions of NLRP3, CASPASE-1, IL-1β, TXNIP and TRX-1 were measured by Western blot analysis. Cells were exposed to Ori (2.5, 5 or 10 μM) for 1 h and then treated with LPS (1 μg/ml) for 1 h or 24 h. a Protein expressions of INOS, HMGB-1, P-P65, P65, IκBα and P- IκBα were measured by Western blot analysis. Cells were exposed to Ori (2.5, 5 or 10 μM) for 1 h and then treated with LPS (1 μg/ml) for 6 h. g Protein expressions of P-Akt, Akt, P-JNK, JNK, Nrf2 and HO-1 were measured by Western blot analysis. b, c, e, f and h Quantification of expressions of previous protein was performed by densitometric analysis, and β-actin acted as an internal control. All results were expressed as the means ± SEM of three independent experiments. *p < 0.05 and **p < 0.01 versus the control group, #p < 0.05 and ##p < 0.01 versus LPS group

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