Fig. 2
From: A piggyBac-based TANGO GFP assay for high throughput screening of GPCR ligands in live cells
The piggyBac-TANGO assays of adenosine A1 receptor (ADORA1) and β1 adrenergic receptor (ADRB1). a NECA induced fluorescence response of ADORA1 in the piggyBac-TANGO assay. b Concentration-response curves of ADORA1 stimulated by NECA in the piggyBac-TANGO assay. c Isoproterenol-induced fluorescence response of ADRB1 in the piggyBac-TANGO assay. d Concentration-response curves of ADRB1 stimulated by isoproterenol in the piggyBac-TANGO assay. Data are presented as the means ± SEM (n = 3). NECA, 5′-N-ethylcarboxamidoadenosine; Isop, isoproterenol. Curves were fitted using GraphPad Prism 6